rnascope 4-plex ancillary kit for multiplex fluorescent reagent kit v2 (Advanced Cell Diagnostics Inc)
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Advanced Cell Diagnostics Inc
rnascope 4-plex ancillary kit for multiplex fluorescent reagent kit v2
Rnascope 4 Plex Ancillary Kit For Multiplex Fluorescent Reagent Kit V2, supplied by Advanced Cell Diagnostics Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rnascope+4-plex+ancillary+kit+for+multiplex+fluorescent+reagent+kit+v2/rnascope+multiplex+fluorescent+reagent+kit+v2/pmc09897724-256-18-9
Average 90 stars, based on 1 article reviews
Rnascope 4 Plex Ancillary Kit For Multiplex Fluorescent Reagent Kit V2, supplied by Advanced Cell Diagnostics Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rnascope+4-plex+ancillary+kit+for+multiplex+fluorescent+reagent+kit+v2/rnascope+multiplex+fluorescent+reagent+kit+v2/pmc09897724-256-18-9
Average 90 stars, based on 1 article reviews
rnascope 4-plex ancillary kit for multiplex fluorescent reagent kit v2 - by Bioz Stars,
2026-09
90/100 stars
Images
1) Product Images from "Spatially resolved transcriptomics reveals pro-inflammatory fibroblast involved in lymphocyte recruitment through CXCL8 and CXCL10"
Article Title: Spatially resolved transcriptomics reveals pro-inflammatory fibroblast involved in lymphocyte recruitment through CXCL8 and CXCL10
Journal: eLife
doi: 10.7554/eLife.81525
Figure Legend Snippet: ( A ) Single-cell RNA sequencing integration with spatial data using ‘anchor’-based integration workflow in Seurat to enable a prediction score for each spot for fibroblast 5. Based on this prediction score, fibroblast 5 is confirmed spatially restricted. ( B ) Multiplex mRNA in situ hybridisation of a representative section of human oral mucosa using specific probes against RAC2 and LCP1 (fibroblast 5 markers), CD14 (macrophages), and CD79A (B cells) to validate in vivo localisation of fibroblast 5 and immune cell populations. Scale bars: 250 µm. ( C ) Schematic illustration of our proposed model summarising pathogenic fibroblast population role in human oral chronic disease. Created with Biorender.
Techniques Used: RNA Sequencing, Multiplex Assay, In Situ, Hybridization, In Vivo
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other:Article Title: Spatially resolved transcriptomics reveals pro-inflammatory fibroblast involved in lymphocyte recruitment through CXCL8 and CXCL10 Article Snippet: Multiplex ISH was performed using kits and probes from Advanced Cell Diagnostics (ACD) which used oligonucleotide probes to RNA targets (RNAscope 4- plex Ancillary Kit for Multiplex Fluorescent Reagent Kit v2; Cat# 323120) as per the manufacturer’s instructions with incubations steps of processed paraffin- embedded tissue performed in Article Title: Spatially resolved transcriptomics reveals pro-inflammatory fibroblast involved in lymphocyte recruitment through CXCL8 and CXCL10 Article Snippet: Multiplex ISH was performed using kits and probes from Advanced Cell Diagnostics (ACD) which used oligonucleotide probes to RNA targets (RNAscope 4-plex Ancillary Kit for Multiplex Fluorescent Reagent Kit v2; Cat# 323120) as per the manufacturer’s instructions with incubations steps of processed paraffin-embedded tissue performed in Formalin-fixed Paraffin-Embedded:Article Title: Single cell derived mRNA signals across human kidney tumors Article Snippet: The Leica DMi8 microscope was used for imaging. .. FFPE tissue sections of 5 μm thickness were processed using a Leica BOND RX to automate staining with the RNAscope Multiplex Fluorescent Reagent Kit v2 Assay and RNAscope 4-plex Ancillary Kit for Staining:Article Title: Single cell derived mRNA signals across human kidney tumors Article Snippet: The Leica DMi8 microscope was used for imaging. .. FFPE tissue sections of 5 μm thickness were processed using a Leica BOND RX to automate staining with the RNAscope Multiplex Fluorescent Reagent Kit v2 Assay and RNAscope 4-plex Ancillary Kit for Article Title: A map of transcriptional heterogeneity and regulatory variation in human microglia Article Snippet: Before staining, slides were directly transferred from −80°C into pre-chilled 4% PFA (methanol-free) in PBS for 45-min and then submerged in boiling ER1 buffer (Advanced Cell Diagnostics, Bio-Techne) for 15-min. After the antigen-retrieval, slides were serially dehydrated through 50%, 70%, 100%, and 100% ethanol for 5 minutes each. .. Tissue sections were then processed using a Leica BOND RX to automate staining with the RNAScope Multiplex Fluorescent Reagent Kit v2 Assay and RNAScope 4-plex Ancillary Kit for RNAscope:Article Title: Single cell derived mRNA signals across human kidney tumors Article Snippet: The Leica DMi8 microscope was used for imaging. .. FFPE tissue sections of 5 μm thickness were processed using a Leica BOND RX to automate staining with the RNAscope Multiplex Fluorescent Reagent Kit v2 Assay and RNAscope 4-plex Ancillary Kit for Article Title: A map of transcriptional heterogeneity and regulatory variation in human microglia Article Snippet: Before staining, slides were directly transferred from −80°C into pre-chilled 4% PFA (methanol-free) in PBS for 45-min and then submerged in boiling ER1 buffer (Advanced Cell Diagnostics, Bio-Techne) for 15-min. After the antigen-retrieval, slides were serially dehydrated through 50%, 70%, 100%, and 100% ethanol for 5 minutes each. .. Tissue sections were then processed using a Leica BOND RX to automate staining with the RNAScope Multiplex Fluorescent Reagent Kit v2 Assay and RNAScope 4-plex Ancillary Kit for Multiplex Assay:Article Title: Single cell derived mRNA signals across human kidney tumors Article Snippet: The Leica DMi8 microscope was used for imaging. .. FFPE tissue sections of 5 μm thickness were processed using a Leica BOND RX to automate staining with the RNAscope Multiplex Fluorescent Reagent Kit v2 Assay and RNAscope 4-plex Ancillary Kit for Article Title: A map of transcriptional heterogeneity and regulatory variation in human microglia Article Snippet: Before staining, slides were directly transferred from −80°C into pre-chilled 4% PFA (methanol-free) in PBS for 45-min and then submerged in boiling ER1 buffer (Advanced Cell Diagnostics, Bio-Techne) for 15-min. After the antigen-retrieval, slides were serially dehydrated through 50%, 70%, 100%, and 100% ethanol for 5 minutes each. .. Tissue sections were then processed using a Leica BOND RX to automate staining with the RNAScope Multiplex Fluorescent Reagent Kit v2 Assay and RNAScope 4-plex Ancillary Kit for Immunohistochemistry:Article Title: Single cell derived mRNA signals across human kidney tumors Article Snippet: The Leica DMi8 microscope was used for imaging. .. FFPE tissue sections of 5 μm thickness were processed using a Leica BOND RX to automate staining with the RNAscope Multiplex Fluorescent Reagent Kit v2 Assay and RNAscope 4-plex Ancillary Kit for |